![]() ![]() Plasmid DNA was quantified in blood samples and mRNA expression for IFN-g in tissue samples. Samples were analysed using quantitative real-time PCR. Peripheral blood and biopsies from the injection site were taken at 13 time points until day 14 post injection (p.i.). Seven grey horses bearing melanoma were injected intratumourally with 250 µg naked plasmid DNA coding for IL-12. The expression of induced interferon gamma (IFN-g) was evaluated in order to determine the pharmacodynamic properties of in vivo gene transduction. Whole blood pharmacokinetics of intratumourally injected naked plasmid DNA coding for equine Interleukin 12 (IL-12) was assessed as a means of in vivo gene transfer in the treatment of melanoma in grey horses. Müller, J-M V Wissemann, J Meli, M L Dasen, G Lutz, H Heinzerling, L Feige, K In vivo induction of interferon gamma expression in grey horses with metastatic melanoma resulting from direct injection of plasmid DNA coding for equine interleukin 12. The study signifies the potential of chitosan nanoparticles as DNA vaccine carrier and adjuvant for effective immunization through non-invasive nasal route. Chitosan nanoparticles thus produced humoral (both systemic and mucosal) and cellular immune responses upon nasal administration. Similarly, cellular responses (cytokine levels) were poor in case of alum adsorbed HBsAg. However, intramuscular administration of naked DNA and alum adsorbed HBsAg did not elicit sIgA titre in mucosal secretions that was induced by nasal immunization with chitosan nanoparticles. ![]() Nasal administration of nanoparticles resulted in serum anti-HBsAg titre that was less compared to that elicited by naked DNA and alum adsorbed HBsAg, but the mice were seroprotective within 2 weeks and the immunoglobulin level was above the clinically protective level. Prepared nanoparticles were characterized for size, shape, surface charge, plasmid loading and ability of nanoparticles to protect DNA against nuclease digestion and for their transfection efficacy. Chitosan p DNA nanoparticles were prepared using a complex coacervation process. This work investigates the preparation and in vivo efficacy of plasmid DNA loaded chitosan nanoparticles for nasal mucosal immunization against hepatitis B. Khatri, Kapil Goyal, Amit K Gupta, Prem N Mishra, Neeraj Vyas, Suresh P Plasmid DNA loaded chitosan nanoparticles for nasal mucosal immunization against hepatitis B. These in vivo findings demonstrate the superior stability of PEGylated polyplexes in physiological milieu and provide important insight on how cationic polymers could be optimized for DNA vaccine delivery. By day 4, co-localization of polyplexes with APCs was observed at the injection site regardless of polymer structure, whereas small amounts of polyplexes were found in the draining lymph nodes. PEGylated polyplexes also distributed more broadly among dermal fibroblasts and allowed greater interaction with antigen-presenting cells (APCs) (dendritic cells and macrophages) starting at around 24 h post-injection. The PEGylated polyplexes had a significantly wider distribution in the tissue than the nonPEGylated polyplexes. However, naked plasmid level dropped to below detection limit after 24 h, whereas polyplexes persisted for up to 2 weeks. Live animal imaging revealed that naked plasmid dispersed quickly in the skin of mice after injection and had a wider distribution than any of the three types of polyplexes. At day 4, however, the polyplexes appeared to result in more transfected skin cells than naked plasmid. Comparable numbers of luciferase expressing cells were observed in the skin of mice receiving naked plasmid or polyplexes one day after transfection. We analyzed transgene expression (luciferase) and the local tissue distribution of the labeled plasmid at the injection site at various time points (from hours to days). To better understand the details of DNA vaccine delivery in vivo, we prepared polymer/ DNA complexes using three structurally distinct cationic polymers and fluorescently labeled plasmid DNA and injected them intradermally into mice. Noelle Zhong, Xiao Panus, David Han, Wenqing Ji, Weihang Wang, ChunĭNA vaccination using cationic polymers as carriers has the potential to be a very powerful method of immunotherapy, but typical immune responses generated have been less than robust. Transgene expression and local tissue distribution of naked and polymer-condensed plasmid DNA after intradermal administration in mice ![]()
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![]() Between 19, 5,195 Phantoms were built in a dozen variants and flown by a dozen nations, making it the most prolific supersonic American warplane ever built. Through the tense Cold War years of the 1960s and 1970s, the Phantom was the symbol of Western tactical airpower. But the F-4 was anything but stealthy or subtle it was a big fighter that muscled its way through combat.Īlong the way, it became one of the most influential aircraft in history. “Phantom” evokes an image of stealth and subtlety, a supernatural nemesis that strikes without warning. If nicknames are destiny, then the F-4 Phantom II fighter was cursed at birth.Įven its official name was ironic. If you know someone who is hooked on flight simulator computer games, then they will absolutely love this fully hydraulic simulated fighter jet experience.Flying Brick. But don't forget, you will be the one in the hot seat, so don't be put off by all the lights, knobs and dials you've got a seriously hefty yet agile jet to get airborne and land again!Īll fighter jet experiences we offer include a full 25 minute pre-flight briefing and you can choose 20, 40 or 60 minutes of flying time, whilst the Dads and Lads experience offers 30 minutes of fighter jet sim action to each of you to give that extra competitive edge to your flying performance. All sessions start with a sign in and meeting your pilot instructor who knows every nook and cranny of this feisty military jet. We are offering a range of fighter jet experiences for you to choose from. The cockpit is the genuine article, right down to the Martin Baker ejector seats and for your fighter jet experience you'll even have the opportunity to try on genuine RAF fighter jet pilot uniforms complete with Mae West life preserver and 'bone dome' flying helmet - impressive stuff! The Mach 2.0 McDonnell Douglas-designed F4 Phantom is one of the most famous fighter jets in the UK and worldwide and this Ferranti Systems simulator has trained many an RAF pilot. This top of the range fighter jet simulator experience offers day and night vision (ideal for top secret flights over the UK), as well as plethora of exciting missions, such as landing and taking off from a aircraft carrier in the sea, or touch and go manoeuvres at military airbases, all accompanied by the sounds and motions of flying a real fighter jet plane. ![]() a 60 minute flight provides enough time to experience day and night landings, aircraft carrier landings, bad weather, air turbulence and even in-flight emergenciesĪlthough it's a simulator, this fighter jet experience is the real deal and one of the best in the world! With a once-operational air force Phantom F4 cockpit being used, you will feel like a proper jet fighter pilot - especially as the high-tech simulator module is mounted on a hydraulic motion platform, so you will move like a fighter jet and sound like one too! ![]() Flights from 20 minutes will give you a real taste of the controls however longer flights will allow a wider range of situations to be simulated e.g.Sessions can usually be tailored to existing flying ability so it doesn't matter if you have never flown before or are an experienced pilot.After the flight you'll receive a de-briefing and be presented with a personalised souvenir certificates. ![]()
![]() Datoĭato is a convenient menu bar utility designed to assist users in managing their schedules, tracking time zones, and receiving full-screen alerts. Additionally, PopClip’s versatility is significantly expanded through its extensive library of extensions. These are just a few examples of the actions that PopClip offers. ![]() Furthuremore, PopClip allows direct web searches based on the selected text, providing instant access to relevant information. PopClip also assists in detecting and correcting spelling errors in the selected text. PopClip provides options to format text with attributes like bold, italic, underline, and more, and facilitates quick translation of selected text, aiding in multilingual tasks. Once you select text, PopClip presents a menu of suggested actions, enabling you to effortlessly choose and execute an action on the selected text.PopClip simplifies these common operations, enhancing text manipulation efficiency. PopClip is a highly effective tool designed to streamline text-related actions on macOS. To top it all off, Soulver seamlessly integrates with macOS, offering support for Dark Mode, Spotlight, Automator, and Terminal functionalities 3. ![]() Users have the freedom to modify the display of numbers and units, incorporate labels and headings into their notes, and organize their calculations into sheets and folders. Not ot mention, Soulver can be personalized to suit individual preferences. Soulver has the ability to understand and execute calculations presented in natural language, enabling queries like “What is 10% of 100?” or “How many hours are in a day?” Soulver also excels at converting between various units, encompassing currencies, weights, distances, temperatures, and more. ![]() ![]() ![]() Continued use of these apps may cause your IP to be blocked indefinitely. This triggers our anti-spambot measures, which are designed to stop automated systems from flooding the site with traffic. Some unofficial phone apps appear to be using GameFAQs as a back-end, but they do not behave like a real web browser does.Using GameFAQs regularly with these browsers can cause temporary and even permanent IP blocks due to these additional requests. If you are using the Brave browser, or have installed the Ghostery add-on, these programs send extra traffic to our servers for every page on the site that you browse, then send that data back to a third party, essentially spying on your browsing habits.We strongly recommend you stop using this browser until this problem is corrected. The latest version of the Opera browser sends multiple invalid requests to our servers for every page you visit.The most common causes of this issue are: MM: Well, I love where I live, and the beach is my happy place! I’m also partial to Sanibel Island-I’ve really loved vacationing there over the years.Your IP address has been temporarily blocked due to a large number of HTTP requests. What is your favorite FL destination or landmark? MM: Make the effort to find more time to create! I got caught up in my NYC advertising career for years, and have been making up for lost time on my personal work ever since I relocated to St Pete. What advice would you give your younger self? MM: I’ve been participating in local group shows this summer at Atelier de Sosi and Five Deuces Galleria, and am really looking forward to exhibiting at the Creative Pinellas Arts Annual this fall. Pete, do you have an upcoming project you’d like to share? Ponce de Leon may have dedicated his life to searching for the Fountain of Youth, but you can easily find it right here at Fairgrounds St. My subject matter is often ripped from the headlines, addressing social issues and frequently referencing the relationship between technology and human behavior. My painting style springboards off the work of classic Pop Artists like James Rosenquist and Tom Wesselman, but with a 21st-century edge by incorporating elements of urban art and social commentary. MM: Artists I admire, as well as current events. What is the most significant influence in your art practice? “Fountain of Youth” is a satire of all that. You find it in the beach culture, the dating scene of the divorcees, the old guys with classic hotrods, women with plastic surgery, etc. A lot of folks migrate here as a kind of second chapter or reboot to their lives up north (myself included), but there’s also the aspect of trying to recapture our youth. MM: Our youth-obsessed culture is well known in America, but it seems especially pronounced in Florida. A quick dip can turn back time in Mark’s FLORIDARAMA, Fountain of Youth. MP: The opportunity for my work to be surrounded by so many other talented artists’ work is what I find most exciting. Pete’s inaugural exhibition is most exciting to you? What about being a part of Fairgrounds St. I always hope viewers take a few extra moments to “look deeper”, appreciate the symbolism and take in the meaning of the piece. It’s fun, quirky and colorful, with some additional subtext. MARK MITCHELL: My piece, “Fountain of Youth,” is a kind of dimensional version of my painting style, Conceptual Pop Art. ![]() What do you want the public to take away from experiencing the FLORIDARAMA diorama you have created for Fairgrounds St. Read on to learn more about what the experience of being part of our inaugural exhibit is like for him! He is also a proud recipient of the Creative Pinellas Emerging Artist Grant 2019-20 and current WADA member. Locally, Mark’s paintings have been awarded at Gasparilla Festival of the Arts, Art Festival Beth-El in St Pete and featured on FOX13’s Good Day Tampa Bay. Mitchell’s work has been exhibited during Miami Art Week’s Scope and Select Art Fairs, shared on SEE | ME digital displays at The Louvre and NYC’s Armory Arts Week, selected by pop-surrealism master Ron English for Baton Rouge Gallery and gained west coast exposure at bG Gallery, Santa Monica. As a Pratt grad, SVA alum and former advertising creative director, he has spent his entire career honing his visual communication skills. Mark Mitchell is an international artist and painter of Conceptual Pop Art. His diorama, Fountain of Youth, is a satirical piece, poking fun at the tendency of so many aging Floridians who attempt to recapture their former youth We recently had the opportunity to interview Fairgrounds St. ![]() ![]() “We brought it to the company, and then there was 2½ years of discussion,” he said, which helped Alabama Power recognize the many benefits of having the IBEW members they already trust take on this work. We want all of NUITF to benefit from this,’” Colston said. We want everyone to come along with us on this and add it to their apprenticeship programs. “Alabama Power reached out to NUITF and said: ‘We’ve already put the criteria together. ![]() ![]() utility companies to boost recruitment and training. NUITF is a joint project of the IBEW and major U.S. Just as significant is the fact that Alabama Power also negotiated adding fiber-optic training to its apprenticeship program with the National Utility Industry Training Fund, Colston said, which will benefit all the utilities and locals that use the NUITF program at IBEW training centers across the U.S. “There are other utility companies that are wanting to build out the middle mile and take advantage of using IBEW workers to do it,” he said, “but they are not as far along as Alabama Power.” “We’re talking with lawmakers on Capitol Hill and policymakers in the Biden administration every day to make sure IBEW members are the ones who will get that work - and, just as importantly, keep it.”Ĭolston said that ongoing efforts to maintain good working relations on the part of labor and management alike has helped move Alabama Power to the head of the pack in this arena. “Broadband and high-speed internet service is on the way to every part of the U.S., especially in rural and traditionally underserved communities,” Keyser said. He and Austin Keyser, assistant to the international president for government affairs, have also been working with the White House and the Edison Electric Institute, which represents investor-owned utilities, on developing a national broadband strategy. “The IBEW lobbied hard to include money in these laws to build out broadband networks,” said Colston. But COVID opened our eyes to the ways that technology has changed, and we started giving thought to broadband work.”Įlectrical utilities are increasingly interested in adding broadband expansion, thanks in part to the billions of dollars in subsidies being made available in recent years under such government funding programs as the American Rescue Plan and the Bipartisan Infrastructure Investment and Jobs Act. “We’ve never really stepped into communications service. “Traditionally, we’ve just been utility linemen, dealing with nothing but electrical wires,” said U-19 Business Manager Casey Shelton. The agreement covers the installation, handling and attaching of fiber-optic cable, plus the intermediate splicing and testing of new-construction fiber. “This is the result of the IBEW lobbying Congress to invest in expanding fiber optic networks and to better allow utilities to build out the middle mile of those networks,” said Utility Department Director Donnie Colston. The pact stands to have far-reaching job-growth and training implications for IBEW utility workers across the U.S. Keep reading for advice on how to answer this question.IBEW utility lineworkers in Alabama will soon be running fiber-optic cabling for broadband internet, thanks to a recent agreement with Alabama Power. You might be wondering how to answer questions about your strengths and weaknesses at your Alabama Power Company interview. What Is Your Greatest Weakness and Greatest Strength? Online Screening ExamĬompanies who require candidates to take assessments are looking to hire applicants who can demonstrate the required skills, be they verbal, numerical, logical, or mechanical skills. Don’t forget to bring your résumé, cover letter, and all related documents. It is a good sign if you are called in for a second interview. You will likely need to face multiple interviews. Interviewĭon’t expect the interview process at Alabama Power Company to be simple. It’s best to use a standard format for your résumé and cover letter and keep the overall length of each document to one page. Have you thought about the format of your résumé, however? Distracting fonts and colors can detract from the professionalism of your application and lead to you being passed over as a candidate. 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Plates and to-go containers are priced by weight, your bill can add up quickly but the food is delicious every time.Ī great place to grab a quick lunch downtown. There are so many delicious options I can never decide, so I get the “Tri-Salad Plate” for variety. Offering “Creative Cuisine To Go”, The Carving Board is a dependable delicatessen and cafe with a wide range of meats, pasta, salads, made-to-order sandwiches and desserts. Stratford Road, Winston-Salem (Thruway Shopping Center) Enjoy Mary’s eclectic salt and pepper collection and Hello Kitty fetish while you’re there!ģ18 S. ![]() Mary’s Diner is only open for dinner the first Friday of each month (Gallery Hop). Hours are Monday-Saturday 8AM-3PM and Sundays 8AM-2PM. Nestled in the heart of the Arts District of Downtown Winston-Salem, Breakfast of Course, Mary’s Gourmet Diner is one of the city’s favorite funky farm-to-table restaurants, focusing on fresh breakfast dishes, often with a twist. Mary’s Gourmet Diner (was Breakfast of Course, Mary’s Too)ħ23 Trade St., Winston-Salem (Downtown/Arts District) Patio seating available in warmer months. The finger-lickin’ barbecue and hushpuppies more than make up for the styrofoam plates. ![]() Housed in an architecturally interesting building that was a Firestone auto shop in the ’60’s, Bib’s Downtown sits at the corner of 5th and Spring Streets, Bib’s specializes in barbecue beef, pork, chicken, turkey and sausages all smoked on-site with plenty of yummy side dishes. Be sure to stay for the live music!Ħ75 West 5th St., Winston-Salem (Downtown) My favorite is their baked brie (pictured) and their fruit and nut salad. It has a wide wine selection and offers tapas, flat bread pizzas, salmon, steak and ribs. 6th Street, Winston-Salem (Downtown/Arts District)Ħth & Vine is an eclectic wine bar. Recently opened in the former Big Shotz/Lucky 32 spot on Stratford Road and aptly named 5 Points according to its location offers upscale American fare often with a Southern slant. Stratford Rd, Winston-Salem (Stratford Rd) There is an abundance of restaurants in Winston-Salem that serve some degree of American food. Winston-Salem Restaurants : Category Listing (Alphabetical)Īmerican restaurants bring together the best of our different regions, ethnic groups and traditions to create something unique and different. And in each category, we’ve included a suggested list of best bets based on quality, price, service and value. Instead, we tried to focus on the independents or local chains. Again, nothing personal, but if you’re staying in a hotel you’ll know about them. The list also leaves out hotel restaurants. It also does not include national chains – you can find them listed in the phone book or with a quick web search. New restaurants open and close every day, sometimes with little notice. The Winston-Salem restaurant list below should not be considered comprehensive. ![]() Interested in Korean, Brazilian or African? You’ll need to get in the car and drive to Greensboro or the Triangle. But if you want Middle Eastern, El Salvadoran, Cuban or Vietnamese, you have about one choice in town. Sure, there is an abundance of Mexican, sushi and Chinese takeout. ![]() What Winston-Salem lacks is a broad range of ethnic and international flavors. Where are the Ethnic and International Flavors? Farm-to-table restaurants that buy local fresh produce from area farms (many organic) are on the rise. There is plenty of traditional American fare from dirt-cheap to surprisingly expensive southern and comfort food restaurants from cafeteria style to white-table cloth establishments and a heavy dose of diners. When seeking Winston-Salem restaurants, you can find examples of almost all the large chains, from steakhouses to international fare to fast-food burger and fries. But if you are willing to get away from the city’s main retail areas, you can find a good range of food choices at moderate prices. To keep the lights on, we receive affiliate commissions via some of our links. ![]() ![]() ![]() NOT: I've been to the museum yesterday. ![]() We CAN'T use the present perfect with a finished time word:.If we are talking about some period of time that began and. We use the present perfect for actions which started in the past and are still happening now OR for finished actions which have a connection to the present. As we can see, then, present perfect and past perfect are similar but with a key difference: present perfect is used for situations leading to the present. Remember that the present perfect is the present tense and the past simple is the past tense.We use the past simple for past events or actions which have no connection to the present.With a finished time word (last week, last month, yesterday):Ĭlick here to download this explanation as a pdf Click here for more information about the present perfect tense Click here for more information about the past simple tense With an unfinished time word (this week, this month, today): It was terrible! (Now there is no result. I 've lost my keys! (The result is that I can't get into my house now).Ī finished action with no result in the present:. ![]()
![]() ![]() ![]() Not only does it bring its own episodes back to life, poke fun at tons of EA properties, and make fun of videogames by mentioning the Xbox 720 and PS4, but there are also some awesome cameos in the game. It would have been fine and dandy if the game simply reconstructed classic episodes and spit them out at gamers to play through but The Simpsons Game goes a step further and actually parodies tons of Hollywood and videogame properties as well. The only thing really missing is a bona fide Treehouse of Horror tribute, though there are elements from the series of Halloween specials throughout the play experience. You'll see such favorites as The Land of Chocolate, Super Happy Fun Fun Homer, Lisa trying to stop lumberjacks from cutting down trees, and several others. Several of everyone's favorite Simpsons episodes are represented quite effectively. Lisa Simpson and Bill Clinton: saxophone masters. While the plot of the game is functional, the real star of The Simpsons Game is the hilarity that ensues. What's inside the manual? Why it's the guide on how to use the family's all-important powers against their captors, of course! Along the way they'll be pitted against villains from the show, EA higher ups (yes, you read that right), and their creator - in more ways than one. Please remember it can take some time for your bank or credit card company to process and post the refund too.The game brings the cartoon household knowingly into their own videogame when Bart stumbles upon a game manual that has seemingly dropped from the heavens. ![]() If approved, you’ll be automatically refunded on your original payment method. We will notify you once we’ve received and inspected your return, and let you know if the refund was approved or not. The fastest way to ensure you get what you want is to return the item you have, and once the return is accepted, make a separate purchase for the new item. Unfortunately, we cannot accept returns on sale items or gift cards. Please get in touch if you have questions or concerns about your specific item. We also do not accept returns for hazardous materials, flammable liquids, or gases. Please inspect your order upon reception and contact us immediately if the item is defective, damaged or if you receive the wrong item, so that we can evaluate the issue and make it right.Ĭertain types of items cannot be returned, like perishable goods (such as food, flowers, or plants), custom products (such as special orders or personalized items), and personal care goods (such as beauty products). You can always contact us for any return question at and issues Items sent back to us without first requesting a return will not be accepted. To start a return, on faulty or incorrect item, you can contact us at If your return is accepted, we’ll send you a return shipping label, as well as instructions on how and where to send your package. Cost of postage will be deducted from refund amount in change of mind cases. You’ll also need the receipt or proof of purchase. To be eligible for a return, your item must be in the same condition that you received it, unopened or unused, with tags, and in its original packaging. We have a 60-day return policy, which means you have 60 days after receiving your item to request a return. ![]() ![]() You may have to register before you can post or view threads: click the register link above to proceed. If this is your first visit, be sure to check out the FAQ by clicking the link above. Using CPS/RM version R17, after a firmware upgrade is performed, modifying any field in RadioProfiles will cause the. A.latest is APX CPS R18.00.04 is now on MOL -The following customer reported issues have been fixed in this release – R18.00.04: Using CPS/RM version R17, after a firmware upgrade is performed, a subsequent export of thecodeplug data will show the incorrect firmware version. FWIW I bought an actual legit APX1500 VHF with O2 head in mint condition, still under Service Essentials, for $650 shipped. Radio Xtractor.Depends on: Control head, accessories, flashcode, encryption, condition, etc. The …Motorola download available for Motorola Solutions APX P25 two-way radios. ASTRO 25 is the next generation of ASTRO digital two-way radio communications by Motorola Solutions., APx API Developer Tools v4. The latest version of MOTOTRBO DepotTool is currently unknown. Motorola Apx Depot Software Motorola Original® batteries are designed to be high-performance and to be as reliable as your radio. Enter promotional price changes into RPM program in .Apx depot software. For APX NEXT, the CPS local programming means you can program small fleets of radios but many of the cloud-connected features and RM-style fleet management will be needing RadioCentral.Communicate efficiently with vendors to manage purchase orders as needed using APX and SPO systems. APX firmware should be available and free if not already. and file appearance is only a few days.RM is still a system and per-unit license fee, as is MotoTRBO firmware services. Also note, that we keep up to date Motorola CPS and Kenwood KPG all the time. It includes MotoTRBO CPS download, MotoTRBO firmware, most recent APX CPS, APX Depot, FlashZap, region free patches, etc. Radio Xtractor.This web site offers biggest radio software collection in Internet. Motorola download available for Motorola Solutions APX P25 two-way radios. The benefit is you do not need multiple versions for each radio (unlike Motorola). The conventional package is more reasonable though is still several hundred dollars. The trunked package is in upwards of $2,500. One package is for conventional programming and the other for trunked programming.Highlighted features include IMPRES Audio Multi-mic noise suppress.Support for the new APX 4000XH portable radio Support for the APX 4000 900 MHz (2 Knob) Support for the APX 1000 900 MHz (2 knob) OTAR and GPS support via V.24 in TXM2000 Support for P25 paging / QCII Encoding tone signaling for APX subscribers APX 6000/7000/XE HAZLOC re-certification (radios updated to the TIA4950 safety standard) XE500 Green with Channel Knob and Xtreme Temperature Cable. ![]() ![]() ![]() It feature an asymmetrical shape to help you find the controls without looking, even while wearing bulky gloves. APX™ XE500 Remote Speaker Microphones are designed with the user’s extreme environment in mind. 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